Surface Plasmon Resonance (SPR)

The P4SPR is a unique portable, 4-channel Surface Plasmon Resonance (SPR) instrument that delivers high-performance measuring and monitoring data of specific targets in complex media such as drugs or disease biomarkers (e.g. antibodies, antigen, hormones) in blood serum or toxic chemicals (e.g. microorganism toxins, industrial organic compounds) in underground waters. The P4SPR's accessible price makes it an attractive label-free system for academic research and startup labs performing assay optimization, system characterization or routine monitoring of proteins and chemicals in lysate, blood, milk or simple buffer. Also, the P4SPR's open architecture makes it easy to combine with other analytical methods such as spectroscopy, chromatography and mass spectrometry to fit your specific needs

Mobility - Small and efficient components

Portable Lightweight (<1.5 kg), textbook-size device (175 mm x 155 mm x 55 mm) and compact optical design for easy portability on the field
Miniature Very small SPR chips (20 mm x 12 mm x 3 mm) and fluidic cell
Low volume < 100 μL injected manually with regular 1-mL syringes for optimal use of sample.
Monitoring Real-time sensorgram display for rapid optimization

Specificity - Relevant information

Low biofouling Patented peptide-based monolayer which has lower nonspecific binding than typical Dextran or PEG monolayers for specific SPR data in complex body fluid samples
Statistics Four-channel acquisition for statistically relevant triplicate measurements and reference data
Large molecules Ideal for direct detection of antibody, protein > 10 kDa or for oligonucleotide in the nM range

Versatility - Combine and conquer

Analytical Open architecture to couple with other analytical methods (e.g. chromatography, spectroscopy, mass spectrometry and electrochemistry)
Software Open source Labview interface for advanced controls and personalized applications
Injection Choice of sample injection: Manual flow-free injection for hour long slow kinetics and low concentrations or continuous syringe pump injection for faster testing
Small molecules Possibility to detect small molecules in blood or cell lysate using sample-preparation free microdialysis SPR

Technical Specifications

Dimensions in mm: 175 x 155 x 55
Weight of < 1.3 kg
USB powered
4-Channel microfluidic cell (min. volume: 150 uL)
Sensitivity of 2750 nm / refractive index unit (typical for gold thin film-based SPR)
Resolution of 1 micro refractive index unit
Dynamic range from 1.30 to 1.39 refractive index unit
Coefficient of variation on signal of > 0.6%
Polychromatic light source
Labview as control software (open source)
Compatible with Ridgeview's TraceDrawer

Protein-Antibody Interactions

Affinité’s technology has been validated with a series of protein- antibody interactions for prostate cancer (PSA / anti-PSA), enzyme detection (lactamase), immunoglobulins and more. It is applicable to ELISA-type assays in a label-free format.

Protein-DNA Interactions

Quantitation of soluble proteins with hybridization of double- stranded DNA fragments functionalized on the SPR chip, monitored step-wise.

Drug-Receptor Interactions

Dose-response curves for small molecule ligands of CD36. Data were fitted to a Langmuir isotherm to determine KD